Additional Cell Culture Reagents and Supplements
STEMCELL Technologies ImmunoCult™ Mouse Treg Differentiation Supplement, Size: 1 mL
ImmunoCult™ Mouse Treg Differentiation Supplement contains a combination of recombinant mouse and human cytokines and a small molecule optimized to promote the differentiation of mouse naïve CD4+ T cells into regulatory T cells (Tregs). This supplement is intended for use with RPMI 1640 Medium (Catalog #36750) containing fetal bovine serum and other additives, as well as anti-mouse CD3 and anti-mouse CD28 monoclonal antibodies as activating agents. This product is recommended for: • Research into the regulation of mouse naïve T cell differentiation and Treg function • Development of procedures to expand and polarize naïve T cells toward a Treg subset
MERCODIA INC Animal Insulin Control, Large Animal, Low Medium High
Animal Insulin Control(10-1221-01)Kit format: 3 x 0.5 mLMeasurement range: low, medium, high.Mercodia Animal Insulin Control (Low, Medium, High) is designed to be used as a three-level control for Mercodia Porcine, Bovine, Ovine, Canine and Equine Insulin ELISA kits.
ILEX LIFE SCIENCES LLC Cellendes 3-D Life Dextran-CD Hydrogel SG Ready-To-Design Kit (1 Kit)
3-D Life is the most flexible system for biomimetic hydrogel design. It uses two polymers that form a hydrogel by covalent crosslinking at ambient temperature. Chemical reaction is non-toxic to cells and occurs rapidly, preventing cell settlement before gel is formed. 3-D Life Dextran-CD Hydrogel SG Kit provides reagents to set up slow gelling, cell-compatible hydrogels for 3D cell cultures. Thiol groups on crosslinker CD-Link form stable thioether bonds with thiol-reactive groups on SG-Dextran, resulting in gel formation. Mixed at physiological pH 7.2 for optimal cell compatibility. CD-Link is designed for MMP cleavage, allowing cell spreading/migration if they express the indicated MMPs. Cell adhesion peptides can be attached to SG-Dextran to provide a cell-adhesive matrix. Dextran hydrogels can be dissolved by adding dextranase, allowing easy cell recovery. One kit allows formation of up to 2 ml hydrogel depending on gel stiffness. Manufactured in Germany by Cellendes GmbH.
ILEX LIFE SCIENCES LLC Cellendes 3-D Life Dextran-HA Hydrogel Ready-To-Design Kit (1 Kit)
3-D Life is the most flexible system for biomimetic hydrogel design for 3D cell cultures. It uses two polymers that form a hydrogel by covalent crosslinking at ambient temperature. The chemical reaction is non-toxic to cells and occurs rapidly, preventing cell settlement before gel is formed. 3-D Life Dextran-HA Hydrogel Kit enables set up of hydrogels containing hyaluronic acid (hyaluronan). Thiol groups on crosslinker HyLink form stable thioether bonds with thiol-reactive groups on SG-Dextran, resulting in gel formation. Mixed at physiological pH 7.2 for optimal cell compatibility. Slow gelation allows for manipulation before onset of gel formation. Cell adhesion peptides (RGD, Cat. No. 09-P-001) can be attached to SG-Dextran to provide a cell-adhesive matrix. Dextran hydrogels can be dissolved by dextranase (Cat. No. D10-1), allowing easy cell recovery. One kit allows formation of 3.3 ml hydrogel of a crosslinking strength of 1.2 mmol/L. Manufactured in Germany by Cellendes GmbH.
CEDARLANE FLAER Alexa Fluor 488 Proaerolysin Variant, 50 μg, Lyophilized Powder, Flow Cytometry
FLAER Alexa Fluor 488 Proaerolysin Variant, 50 μg, Lyophilized Powder, Flow Cytometry
Lifeline Cell Technology Fibroblast Supp. Kit
FibroLife S2 LifeFactors Kit. Supplements for culturing fibroblasts in a 2% FBS environment. Best paired with LM-0001 FibroLife Basal Medium.
Miltenyi Biotec, Inc. CytoStim 0,2ml
Rapid and efficient restimulation of human effector/memory T cells
Stemculture LLC StemCultures LLC StemBeads FGF2 (Trial)
StemBeads FGF2 is a patented growth factor supplement that offers a novel way to culture cells with native Fibroblast Growth Factor 2 more efficiently. FGF2 is key for growth of human induced pluripotent stem cells (iPSCs), fibroblast cells, wound healing cells such as macrophages, cancer cells, and neural progenitor cells (NPCs). StemBeads FGF2 are microbeads composed of an FDA approved, biodegradable PLGA polymer that is loaded with native recombinant human FGF2. Controlled delivery and stable levels overcome the 4.5 hour half-life of FGF2 and improve cell cultures while saving researchers valuable time and resources. StemBeads FGF2 have been tested in media such as mTeSR, mTeSR plus, Stemflex, Flex 8, Nutristem, 10% FBS, and neuronal medium including neural progenitor expansion medium (NPEM) with enhanced cellular profiles. StemBeads FGF2 can be combined with other StemBeads varieties.
InVitria ITS Animal-Free ® - Insulin-Transferrin-Selenium Supplement - 10mLx10
InVitria’s ITS Animal-Free® (Insulin-Transferrin-Selenium) is a recombinant, blood-free cell culture media supplement used to reduce serum and serum-derived components from cell culture media while optimizing cell growth. InVitria offers a family of defined supplements that supply the optimal amounts of the essential protein components in defined, recombinant form: ITS, ITSE, ITSE+A Animal Free®. Our recombinant insulin-transferrin supplements are recommended for use with most cultured cell types, including: Hybridoma, CHO, VERO, HEK293, stem cells, fibroblasts, primary cell isolation and passage, and immortalized cell lines. Made in the USA.
Sigma Aldrich Fine Chemicals Biosciences Hanks' Balanced Salt solution HBSS Modified, with phenol red, without calcium, without magnesium, liquid | 1000ML
Hanks' Balanced Salt solution HBSS Modified, with phenol red, without calcium, without magnesium, liquid | 1000ML
ATHENA ES Bovine Pituitary Extract (BPE), 100 mg
Athena’s Bovine Pituitary Extract is prepared from BSE-free animals. The extracts are prepared from pituitaries harvested from animals in the United States. The pituitaries were obtained only from USDA-inspected facilities where the animals receive pre- and post-mortem inspection and were found to be free of contagious diseases including foot-and-mouth disease, rinderpest, and contagious bovine pleuropneumonia. There are no known cases of bovine spongiform encephalopathy (BSE) in the herds from which the pituitaries were obtained.
ATHENA ES L-Alanyl-L-Glutamine, 0.2M in 0.85% NaCl
L-Alanyl-L-glutamine is a direct replacement for L-glutamine in cell culture media. The alanyl derivative of glutamine is thermally stable, which extends the shelf-life of cell culture medium by limiting deamination of the terminal amino group of the side-chain.